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Gold‐nanoparticle‐coated magnetic beads for concentration and ionization of analytes for laser desorption/ionization mass spectrometry
Author(s) -
Kim MoonJu,
Park JongMin,
Noh JooYoon,
Yun Tae Gyeong,
Kang MinJung,
Pyun JaeChul
Publication year - 2019
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.8372
Subject(s) - chemistry , analyte , mass spectrometry , colloidal gold , nanoparticle , desorption , coating , magnetic nanoparticles , analytical chemistry (journal) , adsorption , chromatography , nanotechnology , organic chemistry , materials science
Rationale Magnetic particles coated with gold nanoparticles (Au NPs) (Au‐MAGs) were developed and used (1) for sample concentration and (2) as a solid matrix for laser adsorption/desorption mass spectrometry (LDI‐MS). Methods The Au‐MAGs were prepared by (1) coating polystyrene on iron oxide NPs (PS‐MNPs), (2) coating poly‐ l ‐lysine on the PS‐MNPs (PLL‐coated PS‐MNPs), and (3) coating negatively charged Au NPs on the PLL‐coated PS‐MNPs (Au‐MAGs). Results The Au‐MAGs were used to concentrate the target analyte by means of electrostatic interactions between the positively charged GHP9 and the negatively charged Au‐MAGs as well as selective interactions (such as gold–sulfur interactions) between glutathione (GSH) and Au‐MAGs. Then, the concentrated analyte could be ionized for LDI‐MS. Conclusions The Au‐MAGs were demonstrated (1) to concentrate the target analyte in a sample solution, tested by electrostatic interactions and selective interactions between gold and sulfur and (2) to ionize the concentrated analyte for LDI‐MS.