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Development and validation of a gas chromatography/tandem mass spectrometry method for simultaneous quantitation of several antipsychotics in human plasma and oral fluid
Author(s) -
Rosado Tiago,
Oppolzer David,
Cruz Belinda,
Barroso Mário,
Varela Samira,
Oliveira Victor,
Leitão Carlos,
Gallardo Eugenia
Publication year - 2018
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.8087
Subject(s) - chemistry , chromatography , levomepromazine , quetiapine , context (archaeology) , gas chromatography/tandem mass spectrometry , therapeutic drug monitoring , gas chromatography–mass spectrometry , antipsychotic , tandem mass spectrometry , haloperidol , mass spectrometry , pharmacology , schizophrenia (object oriented programming) , drug , medicine , psychiatry , psychology , paleontology , biology , dopamine
Rationale Antipsychotic drugs are prescription medications used to treat psychotic disorders, such as schizophrenia, schizoaffective disorder, or psychotic depression. With several antipsychotic drugs currently available all over the world, this class of drugs has quickly gained importance in both the clinical and forensic context. This work describes the development and validation of a methodology for the determination of seven antipsychotic drugs in plasma and oral fluid samples. Methods The antipsychotic drugs (chlorpromazine, clozapine, haloperidol, olanzapine, quetiapine, cyamemazine and, levomepromazine) were isolated from 0.2 mL of oral fluid and 0.5 mL of plasma using solid‐phase extraction (SPE) following analysis by gas chromatography/tandem mass spectrometry (GC/MS/MS). The method was validated according to the international guidelines in terms of selectivity, linearity, accuracy, precision and recovery. Results The procedure was linear within 2–600 ng/mL (plasma) and 2–400 ng/mL (oral fluid), the intervals varying according to the compound; a mean R 2 value of 0.99 was obtained and the calibrator's accuracy (mean relative error) was within a ±15 % interval for all concentrations. The limits of detection ranged from 1 to 10 ng/mL. Within‐ and between‐run precision and accuracy were acceptable for all studied compounds. The extraction efficiency of the process ranged from 79% to 95%. The method was applied to authentic specimens. Conclusions The described method was proven selective and sensitive for the determination of antipsychotics in low sample volumes using SPE and GC/MS/MS. This method was considered suitable not only for routine analysis of patients undergoing antipsychotic treatment (to evaluate compliance), but also in forensic scenarios where the studied compounds may be involved. To the best of our knowledge, this is the first work that reports the determination of antipsychotic drugs in oral fluid using MS/MS.