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Microdialysis sampling combined with ultra‐high‐performance liquid chromatography/tandem mass spectrometry for the determination of geniposide in dialysate of joint cavities in adjuvant arthritis rats
Author(s) -
Wang WenYu,
Wang Wei,
Wu Hong,
Li ShuPing,
Wang Rong,
Dai Li,
Zhang ZhengRong,
Fu Jun,
Deng Ran,
Li Feng
Publication year - 2018
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.8056
Subject(s) - chemistry , paeoniflorin , chromatography , microdialysis , tandem mass spectrometry , mass spectrometry , repeatability , selected reaction monitoring , liquid chromatography–mass spectrometry , electrospray ionization , detection limit , electrospray , high performance liquid chromatography , analytical chemistry (journal) , biochemistry , extracellular
Rationale Microdialysis has been used to detect the concentrations of drugs in tissues. Geniposide (GE), an iridoid glycoside compound, is the main bioactive component of Gardenia jasminoides Ellis fruit. We previously demonstrated that GE could control the activity of cytokines and reduce levels of inflammation in adjuvant arthritis (AA) rats, but the topic of concentration changes over time in the joint synovia of AA has rarely been studied. Methods In this study, a microdialysis technique combined with ultra‐high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry (UHPLC/ESI‐MS/MS) was set up and confirmed to assay GE in the dialysate of the joint cavity in AA rats. Mass detection was conducted in multiple reaction monitoring (MRM) mode with negative ESI, and paeoniflorin (Pae) was used as an internal standard (IS). Results A lower limit of quantitation (LLOQ) of 5 ng/mL was found using this method and with good linearity in the range of 5–4000 ng/mL. All the validation data including accuracy, precision, intra and inter‐day repeatability and stability meet the requirements. The relative recoveries of GE were determined at approximately 40.01%. Conclusions The measurements based on microdialysis combined with UHPLC/ESI‐MS/MS provide a method for sampling and rapid sensitive analysis of GE in the dialysate of the joint cavity in AA rats. This method should be considered for future pharmacokinetics studies.