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Ion suppression effect in desorption electrospray ionization and electrospray ionization mass spectrometry
Author(s) -
Wang Zheng,
Zhu Haijing,
Huang Guangming
Publication year - 2017
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.7977
Subject(s) - chemistry , ion , ionization , ion suppression in liquid chromatography–mass spectrometry , electrospray ionization , analytical chemistry (journal) , electrospray , mass spectrometry , capillary action , ion source , desorption electrospray ionization , chromatography , tandem mass spectrometry , chemical ionization , materials science , organic chemistry , composite material
Rationale Although it is claimed that desorption electrospray ionization (DESI) causes less ion suppression effects than electrospray ionization (ESI), a related investigation with quantification measurement of ion suppression effects is absent. Herein, a comparative analysis of ion suppression effects between DESI and ESI was conducted, with the aiming of quantitatively studying the ion suppression effect. Methods CTVA mixtures, a constant concentration of tioconazole with varied concentrations of atenolol, were analyzed by ESI and DESI. The ion suppression effect was characterized by the signal loss of tioconazole in the mixture compared to the signal intensity of tioconazole without interference (denoted as ( I single − I mixture ) /I single ). According to the variations in the experimental conditions (such as flow rate, solvent composition, substrate material, capillary inner diameter, sheath gas velocity), ion suppression effects in DESI and ESI were compared. Results With the increasing flow rate, the ion suppression effect in DESI became weaker, while the opposite trend was obtained for ESI. As for capillary inner diameter, a smaller inner diameter resulted in weaker ion suppression effects in DESI and ESI. The solvent composition affected the ion suppression effect, and the PTFE substrate presented the weakest ion suppression effect among the five substrate materials. Conclusions Though the ion suppression effect in DESI and ESI was shown to relate to experimental conditions, DESI had less effect than ESI under the same experimental conditions in most cases. Moreover, DESI displayed stronger matrix‐tolerant ability than ESI which is also attributed to the weaker ion suppression effect.