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Lipid profiling of complex biological mixtures by liquid chromatography/mass spectrometry using a novel scanning quadrupole data‐independent acquisition strategy
Author(s) -
Gethings Lee A.,
Richardson Keith,
Wildgoose Jason,
Len Sarah,
Jarvis Sheba,
Bevan Charlotte L.,
Vissers Johannes P.C.,
Langridge James I.
Publication year - 2017
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.7941
Subject(s) - chemistry , analyser , quadrupole time of flight , mass spectrometry , chromatography , quadrupole , lipidomics , data acquisition , profiling (computer programming) , analytical chemistry (journal) , shotgun , tandem mass spectrometry , computer science , biochemistry , physics , atomic physics , gene , operating system
Rationale A novel data‐independent acquisition method is detailed that incorporates a scanning quadrupole in front of an orthogonal acceleration time‐of‐flight (TOF) mass analyser. This approach is described and the attributes are compared and contrasted to other DIA approaches. Methods Specific application of the method to both targeted and untargeted lipidomic identification strategies is discussed, with data from both shotgun and LC separated lipidomics experiments presented. Results The benefits of the fast quadrupole scanning technique are highlighted, and include improvements in speed and specificity for complex mixtures providing high quality qualitative and quantitative data. Conclusions In particular the high specificity afforded by the scanning quadrupole improves qualitative information for lipid identification.