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Rapid microorganism identification with on‐slide proteolytic digestion followed by matrix‐assisted laser desorption/ionization tandem mass spectrometry and database searching
Author(s) -
Yao ZhongPing,
Afonso Carlos,
Fenselau Catherine
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.793
Subject(s) - chemistry , mass spectrometry , chromatography , microorganism , sample preparation in mass spectrometry , matrix assisted laser desorption/ionization , sample preparation , fractionation , matrix (chemical analysis) , tandem mass spectrometry , analytical chemistry (journal) , desorption , electrospray ionization , bacteria , organic chemistry , genetics , biology , adsorption
A simple method for microorganism identification is proposed in this paper. In this method, no isolation or fractionation of microorganisms and no special search algorithm or database are needed. Experimentally, a sample of the unfractionated intact microorganism is subjected to brief on‐slide proteolytic digestion and the digestion product ions detected in matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) are selected for tandem mass spectrometry (MS/MS). Database searching using the MS/MS spectrum identifies the microorganism protein, and thereby, according to the source of the protein, the microorganism. The method was demonstrated to be feasible with enterobacteriophage MS2 as the model microorganism. The novel method is simple, rapid, and requires a small quantity of sample. Copyright © 2002 John Wiley & Sons, Ltd.