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Analysis of non‐derivatised bacteriohopanepolyols by ultrahigh‐performance liquid chromatography/tandem mass spectrometry
Author(s) -
Talbot Helen M.,
Sidgwick Frances R.,
Bischoff Juliane,
Osborne Kate A.,
Rush Darci,
Sherry Angela,
SpencerJones Charlotte L.
Publication year - 2016
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.7696
Subject(s) - chemistry , chromatography , atmospheric pressure chemical ionization , mass spectrometry , selected reaction monitoring , liquid chromatography–mass spectrometry , formic acid , high performance liquid chromatography , triple quadrupole mass spectrometer , tandem mass spectrometry , chemical ionization , analytical chemistry (journal) , ionization , ion , organic chemistry
Rationale Traditional investigation of bacteriohopanepolyols (BHPs) has relied on derivatisation by acetylation prior to gas chromatography/mass spectrometry (GC/MS) or liquid chromatography/MS (LC/MS) analysis. Here, modern chromatographic techniques (ultrahigh‐performance liquid chromatography (UPLC)) and new column chemistries were tested to develop a method for BHP analysis without the need for derivatisation. Methods Bacterial culture and sedimentary lipid extracts were analysed using a Waters Acquity Xevo TQ‐S triple quadrupole mass spectrometer in positive ion atmospheric pressure chemical ionisation (APCI) mode. Waters BEH C18 and ACE Excel C18 were the central columns evaluated using a binary solvent gradient with 0.1% formic acid in the polar solvent phase in order to optimise performance and selectivity. Results Non‐amine BHPs and adenosylhopane showed similar performance on each C18 column; however, BHP‐containing terminal amines were only identified eluting from the ultra‐inert ACE Excel C18 column. APCI‐MS/MS product ion scans revealed significant differences in fragmentation pathways from previous methods for acetylated compounds. The product ions used for targeted multiple reaction monitoring (MRM) are summarised. Conclusions UPLC/MS/MS analysis using an ACE Excel C18 column produced superior separation for amine‐containing BHPs and reduced run times from 60 to 9 min compared with previous methods. Unexpected variations in fragmentation pathways between structural subgroups must be taken into account when optimising MRM transitions for future quantitative studies. Copyright © 2016 John Wiley & Sons, Ltd.