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Speciation of Fe(III)–chelate complexes by electrospray ionization ion trap and laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry
Author(s) -
Neubert Hendrik,
Hider Robert C.,
Cowan David A.
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.756
Subject(s) - fourier transform ion cyclotron resonance , chemistry , electrospray ionization , mass spectrometry , analytical chemistry (journal) , ionization , ion , chelation , aqueous solution , ion cyclotron resonance , desorption , inorganic chemistry , chromatography , cyclotron , adsorption , organic chemistry
Electrospray ionization mass spectrometry (ESI‐MS) was employed as a relatively simple technique to investigate the speciation of complexes formed between Fe(III), the iron chelator 3‐hydroxy‐2‐methyl‐1‐propyl‐1 H ‐pyridin‐4‐one (L) and citrate. The major species in aqueous solution at pH 6.0 of a 1:2:10 molar mixture Fe(III)/L/citrate is the Fe(III)L 2 complex. No evidence for a mixed citrate complex was observed. Furthermore, evidence for the existence of a number of dimeric iron species is presented. All observed species in ESI were singly charged as evidenced by the isotopic envelopes. Laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (LDI‐FTICRMS) provided data that complemented and confirmed the ESI findings. We also demonstrate the possibility of chelated Fe(III) being converted to Fe(II) under ESI and LDI conditions. Copyright © 2002 John Wiley & Sons, Ltd.