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Mass fingerprint analysis of spider mites (Acari) by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry for rapid discrimination
Author(s) -
Kajiwara Hideyuki,
Hinomoto Norihide,
Gotoh Tetsuo
Publication year - 2016
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.7534
Subject(s) - chemistry , mass spectrometry , acari , fingerprint (computing) , chromatography , spider , mass spectrum , matrix assisted laser desorption/ionization , matrix (chemical analysis) , analytical chemistry (journal) , desorption , artificial intelligence , ecology , organic chemistry , adsorption , biology , computer science
Rationale Discrimination of spider mite species is still performed using morphological information, although DNA and other biological approaches have been attempted for identification purposes. These techniques need much time, are expensive, and require specialist staff. As an alternative, matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) analysis is applied for rapid discrimination of spider mite species. Methods Spider mites were analyzed using MALDI‐TOFMS after extraction with 70% formic acid and acetonitrile. A single spider mite was also analyzed directly on double‐sided carbon tape. A dendrogram was compiled from the MS data. Results Evolutionarily close and morphologically similar spider mites, the Kanzawa ( Tetranychus kanzawai ) and the two‐spotted ( T. urticae ) spider mites, as well as three other related species of spider mites, could be discriminated by mass fingerprints. Although female adults were mainly used in this report, male adults and nymphs showed almost the same mass fingerprints and were not considered to affect discrimination capability. A single spider mite on double‐sided carbon tape was analyzed directly by MALDI‐TOFMS. Conclusions Spider mites could be analyzed directly by MALDI‐TOFMS, with evolutionarily and morphologically closely related spider mites showing different mass fingerprints, allowing for their identification. Copyright © 2016 John Wiley & Sons, Ltd.