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Impact of tissue surface properties on the desorption electrospray ionization imaging of organic acids in grapevine stem
Author(s) -
Dong Yonghui,
Guella Graziano,
Franceschi Pietro
Publication year - 2016
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.7495
Subject(s) - chemistry , desorption electrospray ionization , tissue sample , normalization (sociology) , desorption , analytical chemistry (journal) , mass spectrometry imaging , pith , chromatography , mass spectrometry , ion , ionization , adsorption , chemical ionization , biomedical engineering , organic chemistry , medicine , botany , sociology , anthropology , biology
Rationale Desorption electrospray ionization (DESI) imaging is a fast analytical technique used to assess spatially resolved biological processes over unmodified sample surfaces. Although DESI profiling experiments have demonstrated that the properties of the sample surface significantly affect the outcomes of DESI analyses, the potential implications of these phenomena in imaging applications have not yet been explored extensively. Methods The distribution of endogenous and exogenous organic acids in pith and out pith region of grapevine stems was investigated by using DESI imaging, ion chromatography and direct infusion methods. Several common normalization strategies to account for the surface effect, including TIC normalization, addition of the internal standard in the spray solvent and deposition of the standard over the sample surface, were critically evaluated. Results DESI imaging results show that, in our case, the measured distributions of these small organic acids are not consistent with their 'true' localizations within the tissues. Furthermore, our results indicate that the common normalization strategies are not able to completely compensate for the observed surface effect. Conclusions Variations in the tissue surface properties across the tissue sample can greatly affect the semi‐quantitative detection of organic acids. Attention should be paid when interpreting DESI imaging results and an independent analytical validation step is important in untargeted DESI imaging investigations. Copyright © 2016 John Wiley & Sons, Ltd.