Premium
Polyalanine – a practical polypeptide mass calibration standard for matrix‐assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry in positive and negative mode
Author(s) -
Gruendling Till,
Sauerland Volker,
Barahona César,
Herz Corinna,
Nitsch Ute
Publication year - 2016
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.7492
Subject(s) - chemistry , mass spectrometry , analytical chemistry (journal) , calibration , chromatography , tandem mass spectrometry , matrix (chemical analysis) , ionization , selected reaction monitoring , desorption , sample preparation in mass spectrometry , sample preparation , protein mass spectrometry , matrix assisted laser desorption/ionization , ion mobility spectrometry–mass spectrometry , standard addition , electrospray ionization , detection limit , ion , organic chemistry , statistics , mathematics , adsorption
Rationale Mass calibration in matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) is currently obtained using mixtures of individual peptides. This procedure has several drawbacks, including laborious preparation, limited shelf life and unequal calibration mass spacing. Polyalanine, a simple to prepare polydisperse molar mass standard, alleviates these problems. Methods Polyalanine is prepared by the typical protocols for biological sample analytics. Results Polyalanine is the first polymeric standard providing abundant signals in both, positive and negative polarity mode with the typical matrices DHB and HCCA. Conclusions Facile MS as well as MS/MS calibration is thus enabled for the first time in both polarity modes by employing this polydisperse standard. Copyright © 2016 John Wiley & Sons, Ltd.