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Determination of bisphenol A‐glucuronide in human urine using ultrahigh‐pressure liquid chromatography/tandem mass spectrometry
Author(s) -
Hauck Zane Z.,
Huang Ke,
Li Guannan,
Breemen Richard B.
Publication year - 2016
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.7450
Subject(s) - chemistry , chromatography , triple quadrupole mass spectrometer , mass spectrometry , solid phase extraction , tandem mass spectrometry , liquid chromatography–mass spectrometry , metabolite , ion suppression in liquid chromatography–mass spectrometry , selected reaction monitoring , glucuronide , electrospray , urine , detection limit , biochemistry
Rationale Used widely as a plasticizer and as a monomer for plastics, bisphenol A (BPA) is under investigation as a possible endocrine disrupter. As an indication of systemic exposure, a fast and accurate assay was developed for the major BPA metabolite in human urine, BPA‐monoglucuronide (BPA‐G), using ultrahigh‐pressure liquid chromatography/tandem mass spectrometry (UHPLC/MS/MS). Methods Urine samples were prepared using solid‐phase mixed‐mode reversed‐phase/anion‐exchange extraction. BPA‐G was measured using UHPLC/MS/MS with an amide UHPLC column interfaced to a triple‐quadrupole mass spectrometer equipped with negative ion electrospray, collision‐induced dissociation and selected reaction monitoring. [ 13 C 12 ]‐BPA‐G was used as a surrogate standard. Results By measuring the glucuronide metabolite of BPA, potential interference due to BPA contamination from containers, solvents, pipette, etc., was avoided. The standard curve had a linear regression coefficient of 0.999, and the intra‐ and inter‐assay variations were less than 10%. The assay was validated according to FDA guidelines. Conclusions A fast, accurate, and highly selective method for the determination of BPA‐G in human urine was developed and validated using UHPLC/MS/MS. This method is suitable for assessing human exposure to BPA. Copyright © 2016 John Wiley & Sons, Ltd.