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Laser desorption sample transfer for gas chromatography/mass spectrometry
Author(s) -
Seneviratne Chinthaka A.,
Ghorai Suman,
Murray Kermit K.
Publication year - 2015
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.7419
Subject(s) - chemistry , mass spectrometry , desorption , analytical chemistry (journal) , chromatography , solid phase microextraction , laser , gas chromatography , gas chromatography–mass spectrometry , adsorption , physics , organic chemistry , optics
Rationale Ambient mass spectrometry can detect small molecules directly, but complex mixtures can be a challenge. We have developed a method that incorporates small molecule separation based on laser desorption with capture on a solid‐phase microextraction (SPME) fiber for injection into a gas chromatography/mass spectrometry (GC/MS) system. Methods Samples on a metal target were desorbed by a 3 µm mid‐infrared laser focused to a 250 µm spot and 1.2 mJ pulse energy. The desorbed material was aspirated into a metal tube suspended 1 mm above the laser spot and captured on a SPME fiber. The collected material was injected into a GC/MS instrument for analysis. Results We have developed a versatile approach for ambient laser desorption sampling onto SPME for GC/MS analysis. The performance of the laser desorption SPME capture GC/MS system was demonstrated for small molecule standards, a mixture of nitroaromatic explosives, and collected cigarette smoke. Conclusions The utility of ambient laser desorption sampling onto SPME for GC/MS was demonstrated. The performance of the method was evaluated by preparing calibration standards of caffeine over a range from 200 to 1000 ng. Laser desorption ambient sampling of complex mixtures was accomplished using SPME GC/MS. Copyright © 2015 John Wiley & Sons, Ltd.