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Isotope‐labeled differential profiling of metabolites using N ‐benzoyloxysuccinimide derivatization coupled to liquid chromatography/high‐resolution tandem mass spectrometry
Author(s) -
Wagner Michel,
Ohlund Leanne B.,
Shiao Tze Chieh,
Vézina Amélie,
Annabi Borhane,
Roy René,
Sleno Lekha
Publication year - 2015
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.7264
Subject(s) - chemistry , derivatization , chromatography , metabolite , mass spectrometry , reagent , tandem mass spectrometry , amine gas treating , liquid chromatography–mass spectrometry , thiol , metabolomics , resolution (logic) , organic chemistry , biochemistry , artificial intelligence , computer science
Rationale An isotopic labeling strategy based on derivatizing amine‐containing metabolites has been developed using light ( 12 C 6 ) and heavy ( 13 C 6 ) N ‐benzoyloxysuccinimide reagents for semi‐targeted metabolomic applications. Methods Differentially labeled samples were combined and analyzed simultaneously by liquid chromatography/high‐resolution tandem mass spectrometry (LC/HR‐MS/MS) to compare relative amounts of amine‐containing metabolites. The selectivity of the reaction was determined with model metabolites and was shown to also be applicable to thiol and phenol moieties. The potential for relative quantitation was evaluated in cell extracts and the method was then applied to quantify metabolic perturbations occurring in human cultured cells under normal vs. oxidative stress conditions. Results A total of 279 derivatized features were detected in HL60 cell extracts, 77 of which yielded significant concentration changes upon oxidative stress treatment. Based on accurate mass measurements and MS/MS spectral matching with reference standard solutions, 10 metabolites were clearly identified. Derivatized compounds were found to have diagnostic fragment ions from the reagent itself, as well as structurally informative ions useful for metabolite identification. Conclusions This simple derivatization reaction can be applied to the relative quantitation of amine‐, thiol‐ and phenol‐containing compounds, with improved sensitivity and chromatographic peak shapes due to the increased hydrophobicity of polar metabolites not readily amenable to reversed‐phase LC/MS analysis. Copyright © 2015 John Wiley & Sons, Ltd.