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Nylon nanoweb with TiO 2 nanoparticles as a solid matrix for matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
Author(s) -
Kim JoIl,
Ryu SuYeol,
Park JongMin,
Noh JooYoon,
Kang MinJung,
Kwak SeungYeop,
Pyun JaeChul
Publication year - 2014
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.7036
Subject(s) - chemistry , nanoparticle , desorption , mass spectrometry , matrix (chemical analysis) , matrix assisted laser desorption/ionization , ionization , chemical engineering , nanotechnology , chromatography , ion , adsorption , organic chemistry , materials science , engineering
RATIONALE The solid matrices used for matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) enable the analysis of small molecules by preventing fragmentations of organic matrix molecules in the low mass‐to‐charge ratio ( m/z ) range. In this work, a nylon nanoweb with TiO 2 particles was developed as a solid matrix for MALDI‐TOFMS to improve the low intensities of mass peaks, narrow detection ranges and low signal‐to‐noise levels. METHODS The nylon nanoweb with TiO 2 particles was prepared by simultaneously electrospinning a nylon nanoweb and electrospraying TiO 2 nanoparticles measuring 25 nm in diameter to form TiO 2 spheres 300 nm in diameter. RESULTS MS of multiple analytes was demonstrated in the low molecular weight range using eight amino acids. Additionally, leucine‐enkephalin (555.6 g/mol) and cyclic citrullinated peptide (1668 g/mol) were used as model analytes to test the feasibility of a nylon nanoweb containing TiO 2 particles as a solid matrix for MALDI‐TOFMS. CONCLUSIONS The nylon nanoweb with TiO 2 particles can be applied for the detection of volatile small molecule analytes in the m/z ratio range of small molecules. Copyright © 2014 John Wiley & Sons, Ltd.