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Tandem mass spectrometric fragmentation behavior of lignans, flavonoids and triterpenoids in Streblus asper
Author(s) -
Li Chen,
Huang Cuiping,
Lu Tailiang,
Wu Liangdeng,
Deng Shengping,
Yang Ruiyun,
Li Jun
Publication year - 2014
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.7035
Subject(s) - chemistry , fragmentation (computing) , ion trap , mass spectrum , atmospheric pressure chemical ionization , mass spectrometry , triterpenoid , electrospray ionization , tandem mass spectrometry , rutin , chromatography , ion , stereochemistry , chemical ionization , ionization , organic chemistry , operating system , computer science , antioxidant
RATIONALE An unambiguous identification of compounds can be achieved by comparison of known fragmentation patterns. While the literature about fragmentation mechanisms of lignans, flavonoids and triterpenoids is few. So the present study analyses the fragmentation mechanisms of these compounds isolated from Streblus asper.METHODS Electrospray ionization ion trap mass spectrometry (ESI‐ITMS) and atmospheric pressure chemical ionization ion trap mass spectrometry (APCI‐ITMS) were used to obtain the MS n spectra of the compounds . By analyzing the differences between the ions, the fragmentation mechanisms of these compounds were explored. RESULTS Of the 29 compounds detected, 17, 7, and 5 were lignans, flavonoids and triterpenoids, respectively. The majority of lignans were found to give [M – H] – ions of sufficient abundance for MS n analyses. The flavonoids were prone to the loss of CO and H 2 O. The triterpenoids always lost one formic acid molecule and two hydrogens, or one H 2 O from [M – H] – to form the most abundant product ion in the MS n spectrum. CONCLUSIONS ESI/APCI‐ITMS were demonstrated to be fast, effective and practical tools to characterize the structures of flavonoids, triterpenoids and lignans. Results of the present study can help identify the analogous constituents by analyzing their MS n spectra. Copyright © 2014 John Wiley & Sons, Ltd.