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Matrix‐assisted laser desorption/ionization mass spectrometry compatible β‐elimination of O‐linked oligosaccharides
Author(s) -
Huang Yunping,
Konse Tomonori,
Mechref Yehia,
Novotny Milos V.
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.701
Subject(s) - chemistry , chromatography , fetuin , mass spectrometry , sodium hydroxide , aqueous solution , matrix assisted laser desorption/ionization , glycoprotein , matrix (chemical analysis) , sample preparation , fast atom bombardment , sample preparation in mass spectrometry , desorption , biochemistry , electrospray ionization , organic chemistry , adsorption
A new β‐elimination procedure has been introduced to cleave O‐linked oligosaccharides from low‐ to sub‐microgram amounts of glycoproteins prior to analysis by mass spectrometry. Borane‐ammonia complex in aqueous ammonia is used as a cleaving solution alternative to the sodium borohydride/sodium hydroxide medium conventionally used in β‐elimination. The procedure results in minimum sample purification, leading to minimal sample loss and consequently an overall enhancement in sensitivity. It was applied successfully in the analysis of bovine fetuin and submaxillary mucin, as well as to a complex bile‐salt‐stimulated lipase glycoprotein isolated from human milk. Copyright © 2002 John Wiley & Sons, Ltd.