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Microwave extraction–isotope ratio infrared spectroscopy (ME‐IRIS): a novel technique for rapid extraction and in‐line analysis of δ 18 O and δ 2 H values of water in plants, soils and insects
Author(s) -
Munksgaard Niels C.,
Cheesman Alexander W.,
Wurster Chris M.,
Cernusak Lucas A.,
Bird Michael I.
Publication year - 2014
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.7005
Subject(s) - chemistry , analytical chemistry (journal) , extraction (chemistry) , distillation , isotope ratio mass spectrometry , spectroscopy , stable isotope ratio , water vapor , soil water , isotope , calibration , microwave , mass spectrometry , chromatography , soil science , environmental science , physics , statistics , mathematics , quantum mechanics , organic chemistry
RATIONALE Traditionally, stable isotope analysis of plant and soil water has been a technically challenging, labour‐intensive and time‐consuming process. Here we describe a rapid single‐step technique which combines Microwave Extraction with Isotope Ratio Infrared Spectroscopy (ME‐IRIS). METHODS Plant, soil and insect water is extracted into a dry air stream by microwave irradiation within a sealed vessel. The water vapor thus produced is carried to a cooled condensation chamber, which controls the water vapor concentration and flow rate to the spectrometer. Integration of the isotope signals over the whole analytical cycle provides quantitative δ 18 O and δ 2 H values for the initial liquid water contained in the sample. Calibration is carried out by the analysis of water standards using the same apparatus. Analysis of leaf and soil water by cryogenic vacuum distillation and IRMS was used to validate the ME‐IRIS data. RESULTS Comparison with data obtained by cryogenic distillation and IRMS shows that the new technique provides accurate water isotope data for leaves from a range of field‐grown tropical plant species. However, two exotic nursery plants were found to suffer from spectral interferences from co‐extracted organic compounds. The precision for extracted leaf, stem, soil and insect water was typically better than ±0.3 ‰ for δ 18 O and ±2 ‰ for δ 2 H values, and better than ±0.1 ‰ for δ 18 O and ±1 ‰ for δ 2 H values when analyzing water standards. The effects of sample size, microwave power and duration and sample‐to‐sample memory on isotope values were assessed. CONCLUSIONS ME‐IRIS provides rapid and low‐cost extraction and analysis of δ 18 O and δ 2 H values in plant, soil and insect water (≈10–15 min for samples yielding ≈ 0.3 mL of water). The technique can accommodate whole leaves of many plant species. Copyright © 2014 John Wiley & Sons, Ltd.