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Determination of glutathione in spruce needles by liquid chromatography/tandem mass spectrometry
Author(s) -
Guček Marjan,
Makuc Simon,
Mlakar Anita,
BeričnikVrbovšek Julija,
Marsel Jože
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.700
Subject(s) - chemistry , chromatography , mass spectrometry , glutathione , tandem mass spectrometry , electrospray , selected reaction monitoring , triple quadrupole mass spectrometer , electrospray ionization , ion trap , organic chemistry , enzyme
Abstract For the determination of glutathione (GSH) and its oxidized form (GSSG) in spruce needles their electrospray mass and MS/MS spectra were recorded with an ion trap mass spectrometer (ITMS, LCQ, Finnigan) and a triple stage quadrupole mass spectrometer (TSQ, Quattro II, Micromass). A study of the stability of GSH in aqueous solutions shows the presence of dimeric and trimeric forms of GSH, as well as GSSG, GSH‐sulfonate and GSH‐sulfinic acid. The same components were also found in extracts of spruce needles. We developed an assay which is suitable for monitoring low concentrations of GSH and similar compounds in plant tissues, employing the sensitivity and specificity of LC/MS/MS. Preliminary results on the mass spectrometric determination of GSH in spruce needles are given. Copyright © 2002 John Wiley & Sons, Ltd.