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Analysis of protein ions in the range 3000–12000 Th under partial (no discharge) atmospheric pressure chemical ionization conditions using ion trap mass spectrometry
Author(s) -
Cristoni Simone,
Bernardi Luigi Rossi,
Biunno Ida,
Guidugli Federico
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.693
Subject(s) - atmospheric pressure chemical ionization , chemistry , mass spectrometry , ion trap , analytical chemistry (journal) , ion , chemical ionization , chromatography , ionization , myoglobin , quadrupole ion trap , atmospheric pressure , organic chemistry , oceanography , geology
Abstract A new approach, based on the use of atmospheric pressure chemical ionization ion trap mass spectrometry (APCI‐ITMS), but without a corona discharge, was investigated for application to creating and monitoring protein ions. It must be emphasized that APCI is not usually used in protein analysis. In order to verify the applicability of the proposed method to the analysis of proteins, two standard proteins (horse cytochrome c and horse myoglobin) were analyzed. A mixture of the two proteins was also analyzed showing that this novel approach, based on the use of APCI, can be used in the analysis of protein mixtures. Copyright © 2002 John Wiley & Sons, Ltd.