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Identification of hemes and related cyclic tetrapyrroles by matrix‐assisted laser desorption/ionization and liquid secondary ion mass spectrometry
Author(s) -
Youn HyungSun,
Burkhalter Robert S.,
Timkovich Russell
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.689
Subject(s) - chemistry , mass spectrometry , tetrapyrrole , fast atom bombardment , analytical chemistry (journal) , matrix assisted laser desorption/ionization , matrix (chemical analysis) , sample preparation in mass spectrometry , chemical ionization , matrix assisted laser desorption electrospray ionization , chromatography , desorption , ionization , ion , electrospray ionization , thermal ionization mass spectrometry , organic chemistry , adsorption , enzyme
Mass spectrometry has proven to be a powerful technique applicable on trace amounts for the identification of known hemes and cyclic tetrapyrroles, and for providing critical information for the structure of new and novel versions. This report describes investigations of the practical limits of detection for such bioinorganic prosthetic groups, primarily by liquid secondary ion mass spectrometry (LSIMS) and matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS), including a survey of the utility of common matrices. The lower limit of detection under favorable conditions extends to low picomole amounts. Certain derivatization techniques, such as methyl esterification and chelation to zinc, both increase the sensitivity of analyses and provide spectroscopic signatures that enable heme/cyclic tetrapyrrole ions to be identified in the presence of contaminants. Copyright © 2002 John Wiley & Sons, Ltd.