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Dual track time‐of‐flight mass spectrometry for peptide quantification with matrix‐assisted laser desorption/ionization
Author(s) -
Bae Yong Jin,
Moon Jeong Hee,
Kim Myung Soo
Publication year - 2014
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.6845
Subject(s) - chemistry , mass spectrometry , detector , ion , ionization , time of flight mass spectrometry , tandem mass spectrometry , matrix assisted laser desorption/ionization , matrix (chemical analysis) , analytical chemistry (journal) , chromatography , desorption , optics , physics , organic chemistry , adsorption
RATIONALE Previously, we reported a method ( Anal. Chem . 2012 , 84 , 10332) for peptide quantification based on matrix‐assisted laser desorption/ionization (MALDI) time‐of‐flight (TOF) mass spectrometry (MS). In the method, the peptide‐to‐matrix ion abundance ratio was utilized. Implementation of the method with a commercial MALDI‐TOF can be somewhat inconvenient because matrix‐derived ions are routinely deflected away to avoid detector saturation. A solution for this inconvenience is required. METHODS We installed a detector to acquire the TOF spectrum of the ions thrown away to avoid detector saturation. By sending the matrix‐ and peptide‐derived ions along two different tracks and detecting them with different detectors, the inconvenience mentioned above could be avoided. RESULTS Excellent linearity of the calibration curves obtained by the dual track TOF spectrometry is demonstrated. The method also allows for the acquisition of the tandem mass spectrum of a selected peptide, which can be useful for its identification. CONCLUSIONS We devised the dual track MALDI‐TOF MS method to avoid detector saturation and demonstrated that the quantification and identification of peptides can be performed simultaneously. Copyright © 2014 John Wiley & Sons, Ltd.