Premium
Influence of the eluent composition on the ionization efficiency for morphine of pneumatically assisted electrospray, atmospheric‐pressure chemical ionization and sonic spray
Author(s) -
Dams Riet,
Benijts Tom,
Günther Wolfgang,
Lambert Willy,
Leenheer André De
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.683
Subject(s) - atmospheric pressure chemical ionization , chemistry , electrospray ionization , ionization , chromatography , chemical ionization , desorption electrospray ionization , mass spectrometry , column chromatography , analytical chemistry (journal) , electrospray , ion source , direct electron ionization liquid chromatography–mass spectrometry interface , high performance liquid chromatography , ion , organic chemistry
A comparative study of three atmospheric‐pressure ionization (API) sources for liquid chromatography/mass spectrometry (LC/MS), namely pneumatically assisted electrospray or ionspray (IS), atmospheric‐pressure chemical ionization (APCI), and sonic spray (SS), with respect to the influence of the eluent composition on the ionization of morphine, is presented. The effect of organic modifiers, volatile acids, and buffer systems (with and without pH adjustment) in the LC mobile phase on the ionization efficiency of each interface is described. We conclude that for all three ion sources, the composition of the liquid phase had a serious impact on the ionization of the target compound. For IS and SS, very similar behavior towards the LC eluent was observed. In both cases, an increase in organic modifier resulted in an increase in ionization, while an increasing amount of volatile acid or buffer caused signal suppression. APCI, on the other hand, proved to respond completely differently towards the changes in the eluent. Again, an increased ionization was observed with an increase in organic modifier content but this time also in the presence of mobile phase additives like acids or buffers. Finally, we concluded that APCI proved to be the preferred ion source for the test compound because of its robust character and its direct applicability in traditional LC analysis. Copyright © 2002 John Wiley & Sons, Ltd.