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Electric‐field‐enhanced collection of laser‐ablated materials onto a solvent bridge for electrospray ionization mass spectrometry
Author(s) -
Kaufman Eric,
Smith Whitney,
Kowalski Matthew,
Beech Iwona,
Sunner Jan
Publication year - 2013
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.6600
Subject(s) - chemistry , mass spectrometry , electric field , desorption electrospray ionization , electrospray ionization , analytical chemistry (journal) , ionization , mass spectrometry imaging , ion mobility spectrometry , laser , interfacing , solvent , electrospray , ambient ionization , chromatography , ion , chemical ionization , optics , organic chemistry , physics , quantum mechanics , political science , law
RATIONALE Ambient imaging mass spectrometry methods are critically dependent on the ability to efficiently collect all substances from a well‐defined area of the sample. Improvements in this area are critical and enabling. METHODS Methods for the efficient collection of laser‐ablated materials directly into a solvent, for immediate transport to an ion source, have been explored using the application of electric fields. RESULTS Electric‐field‐enhanced collection of laser‐ablated materials has been demonstrated. Demonstrated increases in collection efficiency are as large as two orders of magnitude, in particular for hydrated biological materials, such as living bacterial colonies. This was achieved by applying approximately 1 kV between the sample and the receiving solvent surface. CONCLUSIONS Electric‐field‐enhanced collection of laser‐ablated materials holds great promise for ambient sampling and imaging mass spectrometry with rapid and direct interfacing to ionization sources, such as electrospray. Copyright © 2013 John Wiley & Sons, Ltd.