Premium
Determination of 13 C natural abundance of amino acid enantiomers in soil: methodological considerations and first results
Author(s) -
Glaser Bruno,
Amelung Wulf
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.650
Subject(s) - chemistry , enantiomer , derivatization , amino acid , alanine , chromatography , detection limit , valine , isotope ratio mass spectrometry , mass spectrometry , stereochemistry , biochemistry
The application of a combined gas chromatography‐combustion/isotope ratio mass spectrometry (GC‐C/IRMS) method for stable carbon isotope analysis of amino acid enantiomers in soil samples is presented. Triplicate δ 13 C analyses of pentafluoropropionyl (PFP) isopropyl ester derivatives of 27 amino acid enantiomers revealed that discrimination of 13 C during derivatization is different for different amino acid enantiomers and different amounts. Injection of increasing amounts of amino acid derivatives showed that the isotopic signal varied up to 10‰ for D‐aspartic acid. Correction for the δ 13 C signal of underivatized amino acid enantiomers is possible for all investigated amino acid enantiomers using logarithmic functions. Operating the GC‐C/IRMS system in the split‐mode (split ratio 1:12) is possible but resulted in a higher isotopic discrimination. The detection limit approached 3 ng for some amino acid enantiomers in the splitless mode, while the lower limit of routine determination exceeded 10 ng injection amount. The upper limit at which accurate stable isotope values were obtained was 200 ng injection amount. Compound‐specific δ 13 C analysis of alanine, valine, aspartic and glutamic acid showed that the D‐forms were enriched in 13 C relative to the L‐forms, suggesting that microbes significantly contributed to the formation of the D‐enantiomers in soil. Copyright © 2002 John Wiley & Sons, Ltd.