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Highly robust stainless steel tips as microelectrospray emitters
Author(s) -
Ishihama Yasushi,
Katayama Hiroyuki,
Asakawa Naoki,
Oda Yoshiya
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.647
Subject(s) - chemistry , electropolishing , capillary action , chromatography , capillary electrophoresis , mass spectrometry , fabrication , analytical chemistry (journal) , common emitter , volumetric flow rate , composite material , materials science , optoelectronics , electrode , medicine , alternative medicine , physics , pathology , quantum mechanics , electrolyte
Tapered stainless steel spray tips for sheathless microelectrospray ionization (µESI) have been developed. The fabrication procedure for the tapered stainless steel tips was optimized using an electropolishing technique followed by removal of the burr. Using the tip as the µESI emitter, a stable ESI spray was obtained at a flow rate of 20 nL/min. The sensitivity of the µESI system was almost two orders greater than that of the conventional ion spray system. The tip was highly stable, and was successfully used for over 1000 h. Moreover, these stainless steel tips were suitable for use with sheathless capillary electrophoresis/mass spectrometry (CE/MS) and capillary liquid chromatography/mass spectrometry (LC/MS) for routine analysis in proteomic and pharmaceutical applications. Copyright © 2002 John Wiley & Sons, Ltd.