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Platinum vapor deposition surface‐assisted laser desorption/ionization for imaging mass spectrometry of small molecules
Author(s) -
Kawasaki Hideya,
Ozawa Tomoyuki,
Hisatomi Hirotaka,
Arakawa Ryuichi
Publication year - 2012
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.6301
Subject(s) - chemistry , mass spectrometry , maldi imaging , analytical chemistry (journal) , mass spectrometry imaging , deposition (geology) , analyte , desorption , chemical vapor deposition , layer (electronics) , nanoparticle , matrix assisted laser desorption/ionization , chromatography , nanotechnology , adsorption , materials science , organic chemistry , paleontology , sediment , biology
RATIONALE Matrix‐assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) allows for the simultaneous detection and imaging of several molecules in a sample. However, when using an organic matrix in the MALDI‐IMS of small molecules, inhomogeneous matrix crystallization may yield poorly reproducible peaks in the mass spectra. We describe a solvent‐free approach that employs a homogeneously deposited metal nanoparticle layer (or film) for small‐molecule detection. METHODS Platinum vapor deposition surface‐assisted laser desorption/ionization imaging mass spectrometry (Pt vapor deposition SALDI‐IMS) of small molecules was performed as a solvent‐free and organic‐matrix‐free method. A commercially available magnetron sputtering device was used for Pt deposition. Vapor deposition of Pt produced a homogenous layer of nanoparticles over the surface of the target imaging sample. RESULTS The effectiveness of Pt vapor deposition SALDI‐IMS was demonstrated for the direct detection of small analytes of inkjet ink on printed paper as well as for various other analytes (saccharides, pigments, and drugs) separated by thin‐layer chromatography (TLC), without the need for extraction or concentration processes. The advantage of choosing Pt instead of Au in SALDI‐IMS was also shown. CONCLUSIONS A solvent‐free approach involving the direct deposition of Pt on samples (SALDI‐IMS) is effective for the analysis of inkjet‐printed papers and various analytes separated by TLC. This method would be useful in imaging analyses of various insulating materials such as polymers and biological materials. Copyright © 2012 John Wiley & Sons, Ltd.