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The evaluation and implementation of direct analysis in real time quadrupole time‐of‐flight tandem mass spectrometry for characterization and quantification of geniposide in Re Du Ning Injections
Author(s) -
Li YanJing,
Wang ZhenZhong,
Bi YuAn,
Ding Gang,
Sheng LongSheng,
Qin JianPing,
Xiao Wei,
Li JiaChun,
Wang YongXiang,
Wang Xue
Publication year - 2012
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.6235
Subject(s) - dart ion source , chemistry , chromatography , mass spectrometry , analyte , repeatability , quadrupole time of flight , sample preparation , tandem mass spectrometry , time of flight mass spectrometry , analytical chemistry (journal) , dart , ionization , ion , electron ionization , organic chemistry , computer science , programming language
RATIONALE The Direct Analysis in Real Time (DART) ionization source coupled with a quadrupole time‐of‐flight tandem mass spectrometry (Q‐TOF MS/MS) system has the capability to desorb analytes directly from samples from complex Chinese herbal preparations without sample cleanup or chromatographic separation. METHODS In this work, a method based on DART/Q‐TOF MS/MS has been developed for rapid determination of geniposide present in 'Re Du Ning Injections', a Chinese herbal preparation. The method has been evaluated for both qualitative and quantitative analysis of geniposide in Re Du Ning Injections. RESULTS Variables including polarity for ion detection, DART gas heater temperature, matrix effect and sample presentation speed were investigated. The quantitative method was validated with respect to linearity, sensitivity, repeatability, precision and accuracy by using both internal and external standards. A comparison of the results obtained using the DART‐based method was made with those obtained using a conventional High‐Performance Liquid Chromatography/Diode‐Array Detector (HPLC/DAD) by analyzing geniposide in four batches of Re Du Ning Injections. CONCLUSIONS The DART/Q‐TOF MS/MS‐based method provides a rapid, efficient and powerful method to analyze compounds from complex Traditional Chinese Medicines with limited sample preparation thus reducing time and complexity of quality control for those materials. Copyright © 2012 John Wiley & Sons, Ltd.