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Gas chromatography/negative‐ion chemical ionisation mass spectrometry for the quantitative analysis of morphine in human plasma using pentafluorobenzyl carbonate derivatives
Author(s) -
Leis H. J.,
Windischhofer W.,
Fauler G.
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.618
Subject(s) - chemistry , chromatography , mass spectrometry , chemical ionization , morphine , gas chromatography , gas chromatography–mass spectrometry , calibration curve , quantitative analysis (chemistry) , trimethylsilyl , analytical chemistry (journal) , ion , ionization , detection limit , medicinal chemistry , organic chemistry , medicine , pharmacology
A sensitive and specific method for the quantitative determination of morphine in human plasma is presented. Morphine was extracted from plasma by solid phase extraction on C18 and converted to its pentafluorobenzyl carbonate trimethylsilyl derivative. The derivatives were analysed without further purification. Using gas chromatography/negative ion chemical ionisation mass spectrometry, a useful diagnostic fragment ion at m/z 356 is obtained at high relative abundance. Deuterated morphine was used as internal standard. Calibration graphs were linear within the range 1.25 to 320 nmol/L. Intra‐day precision was 3.82% (15 nmol/L), 2.85% (75 nmol/L) and 4.13% (225 nmol/L), inter‐day variability was found to be 1.77% (15 nmol/L), 4.95% (75 nmol/L) and 9.88% (225 nmol/L). Inter‐day accuracy showed deviations of 2.18% (15 nmol/L), −0.72% (75 nmol/L) and −0.13% (225 nmol/L). The method is rugged and robust and has been applied to the batch analysis of morphine during pharmacokinetic profiling of the drug. Copyright © 2002 John Wiley & Sons, Ltd.