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Iberian ham typification by direct infusion electrospray and photospray ionization mass spectrometry fingerprinting
Author(s) -
GonzálezDominguez R.,
GarcíaBarrera T.,
GómezAriza J.L.
Publication year - 2012
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.6155
Subject(s) - chemistry , mass spectrometry , chromatography , analytical chemistry (journal) , tandem mass spectrometry , electrospray ionization , mass spectrum , electrospray
RATIONALE Iberian ham is a product of high commercial value whose quality mainly depends on breeding and feeding of pigs in an authorized way. Simple, fast, simple, reliable and high‐throughput analytical methods are necessary to assure the quality of ham and for fraud prevention. Tandem mass spectrometry (MS/MS) is proposed as an advantageous alternative over other analytical techniques commonly used in this industry for product authentication. METHODS The analytical approach is based on direct infusion electrospray mass spectrometry (ESI(+)‐MS) of dichloromethane/methanol (60:40) extracts of ham intramuscular fat. Similarly, atmospheric pressure photoionization ionization mass spectrometry (APPI(+)‐MS) was used with a flow injection analysis system for sample introduction with methanol/water (50%) as the mobile phase and toluene as the dopant. All experiments were performed on an API QSTAR® XL Hybrid system using both ESI and APPI sources. RESULTS The ESI(+)‐MS mass spectra present several clusters of peaks attributed to ammonium adducts [M + NH 4 + ] of lipid compounds (mono‐, di‐ and triacylglycerols – MGs, DGs, TGs, and free fatty acids – FFAs), that can be identified by MS/MS spectra. On the other hand, the APPI(+)‐MS spectra present [M + H + ] ions and reflect a higher fragmentation of the sample. Five different types of Iberian ham samples were successfully classified using partial least‐squares discriminant analysis (PLS‐DA) of data from these samples. CONCLUSIONS The application of direct infusion tandem mass spectrometry to dichloromethane/methanol extracts from intramuscular fat ham allows the simple, fast and reliable fingerprinting typification of different Iberian ham samples from pigs with different diets. With the proposed method, sample handling is minimal and chromatographic separation is not necessary, which represents an evident advantage over other analytical procedures usually used for this purpose. Copyright © 2012 John Wiley & Sons, Ltd.