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Dried blood spot (DBS) sample collection for determination of the oxidative stress biomarker 8‐epi‐PGF 2α in humans using liquid chromatography/tandem mass spectrometry
Author(s) -
Bastani Nasser E.,
Gundersen Thomas E.,
Blomhoff Rune
Publication year - 2012
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.6149
Subject(s) - chemistry , chromatography , dried blood spot , tandem mass spectrometry , detection limit , liquid chromatography–mass spectrometry , mass spectrometry , solid phase extraction , isoprostanes , oxidative stress , lipid peroxidation , biochemistry
RATIONALE F2‐isoprostanes are a series of prostaglandin F2‐like compounds that are formed by free‐radical‐catalyzed peroxidation of arachidonic acid (ARA). Several F2‐isoprostanes, but in particular 8‐epi‐PGF 2α , are widely used as oxidative stress biomarkers. In this study we have developed an analytical tool for finger‐tip blood sampling and analysis of 8‐epi‐PGF 2α from dried blood spots (DBS). METHODS We have applied solid‐phase extraction (SPE) and liquid chromatography/tandem mass spectrometry (LC/MS/MS) for the extraction, separation and detection of 8‐epi‐PGF 2α in DBS and have studied the stability of this marker using the DBS collection platform. RESULTS The mass limit of detection (mLOD) for 8‐epi‐PGF 2α extracted from DBS samples was 1.5 pg while the concentration limit of detection (cLOD) and concentration limit of quantitation (cLOQ) were 6 pg/mL and 18 pg/mL, respectively. All values based on triplicate analysis. Sufficient stability of 8‐epi‐PGF 2α in DBS was achieved by preconditioning DBS paper with vitamin E and BHT. CONCLUSIONS The developed method is sensitive, specific, robust, efficient, and can accurately measure endogenous concentrations of 8‐epi‐PGF 2α in DBS. Thus, it offers an analytical approach to measure 8‐epi‐PGF 2α by a novel sample collection technique that is less invasive and costly than conventional techniques. Copyright © 2012 John Wiley & Sons, Ltd.