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Generic method for on‐line extraction of drug substances in the presence of biological matrices using turbulent flow chromatography
Author(s) -
Herman J. L.
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.593
Subject(s) - adme , chemistry , chromatography , mass spectrometry , sample preparation , analyte , throughput , biochemical engineering , computer science , biochemistry , telecommunications , engineering , in vitro , wireless
The use of liquid chromatography/mass spectrometry (LC/MS) to quantify drugs in biological matrices has been well established over the last decade. Extremely fast LC/MS methods are commonplace in the pharmaceutical industry for high‐throughput Absorption, Distribution, Metabolism and Excretion (ADME) screening. However, to truly take full advantage of high‐throughput ADME screening, a generic method is needed that eliminates the need to develop a new method for each new compound being screened. New developments in the stationary phase of turbulent flow columns has allowed us to develop an on‐line biological sample cleanup method that is suitable for over 99% of the compounds in the Cephalon database. Copyright © 2002 John Wiley & Sons, Ltd.