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Identification of the interface of a large protein–protein complex using H/D exchange and Fourier transform ion cyclotron resonance mass spectrometry
Author(s) -
Yamada Naoyuki,
Suzuki Eiichiro,
Hirayama Kazuo
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.579
Subject(s) - infrared multiphoton dissociation , chemistry , fourier transform ion cyclotron resonance , hydrogen–deuterium exchange , mass spectrometry , deuterium , ion cyclotron resonance , analytical chemistry (journal) , dissociation (chemistry) , ion , tandem mass spectrometry , chromatography , atomic physics , cyclotron , physics , organic chemistry
An infrared multiphoton dissociation (IRMPD) spectrum, obtained by Fourier transform ion cyclotron resonance mass spectrometry (FTICRMS), was used to dissociate and to identify fragment ions from recombinant human interleukin‐6 (IL‐6; 21 KDa). The entire sequence was assigned by a single IRMPD experiment, and the observed fragment ions reflected the IL‐6 secondary structure. This method was combined with H/D off‐exchange to identify IL‐6 and anti‐human IL‐6 mouse monoclonal antibody MH166 (150 kDa) binding sites in the IL‐6 molecule. To facilitate the data analysis, the protein complex formation and the hydrogen exchange were performed with an immobilized antibody. Quenching of the hydrogen exchange reaction and collection of the deuterated IL‐6 were performed by elution under acidic conditions to measure the mass spectrum directly. IL‐6 was dissociated by using IRMPD, and the interface of IL‐6 bound to anti‐IL‐6 antibody MH166 was determined to analyze the deuterium incorporation level of each fragment ion. Thus, two discontinuous regions, Leu 126‐Lys 131 and Asp 160‐Met 184, were identified as the antibody binding sites. These regions are adjacent to each other on the tertiary structures determined by NMR and X‐ray analyses. Copyright © 2002 John Wiley & Sons, Ltd.