Premium
On‐target fraction collection for the off‐line coupling of capillary isoelectric focusing with matrix‐assisted laser desorption/ionization mass spectrometry
Author(s) -
Chartogne Anne,
Gaspari Marco,
Jespersen Sonja,
Buscher Brigitte,
Verheij Elwin,
Heijden Robert van der,
Tjaden Ubbo,
Greef Jan van der
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.566
Subject(s) - chemistry , mass spectrometry , isoelectric focusing , chromatography , analytical chemistry (journal) , capillary electrophoresis–mass spectrometry , maldi imaging , desorption , matrix assisted laser desorption/ionization , capillary action , ionization , matrix (chemical analysis) , mass spectrum , electrospray ionization , ion , adsorption , biochemistry , materials science , organic chemistry , composite material , enzyme
In the present work, we describe a collection system for the off‐line coupling of capillary isoelectric focusing (CIEF) with matrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐TOF) mass spectrometry. In this system, the capillary effluent is directly deposited in fractions onto the MALDI target via the use of a sheath liquid. The collected fractions are subsequently supplemented with matrix and further analysed by MALDI‐TOF mass spectrometry for mass assignment. The experimental set‐up includes a fiber optic based UV detector operating at 280 nm, which allows the study of the influence of the sheath liquid composition on the CIEF separation. The influence of the carrier ampholyte concentration on the protein MALDI spectra was also evaluated and the feasibility of the collection method was finally demonstrated with a mixture of four standard proteins. Copyright © 2002 John Wiley & Sons, Ltd.