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In vitro metabolite identification using fast gradient high performance liquid chromatography combined with tandem mass spectrometry
Author(s) -
Hop Cornelis E. C. A.,
Tiller Phillip R.,
Romanyshyn Leslie
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.556
Subject(s) - chromatography , mass spectrometry , chemistry , metabolite , tandem mass spectrometry , high performance liquid chromatography , triple quadrupole mass spectrometer , tandem , liquid chromatography–mass spectrometry , selected reaction monitoring , analytical chemistry (journal) , materials science , biochemistry , composite material
Metabolite identification can provide critical information for further synthesis during the lead optimization stage of drug discovery. Fast chromatographic gradient methodology was explored to reduce the analysis time for metabolite identification by liquid chromatography/tandem mass spectrometry while maintaining chromatographic separation of all components of interest. Short HPLC columns (2 cm) containing 3 µm particles were used and the chromatographic conditions involved elevated flow rates (1.0–2.5 mL/min) and fast, full range linear gradients (<2 min). Detection was accomplished by fast scanning triple quadrupole mass spectrometers or a quadrupole time‐of‐flight mass spectrometer. Analysis of liver microsomal incubations of several compounds indicates that separation of isomeric metabolites as well as the parent compound is feasible with analysis times not exceeding 2 minutes. Copyright © 2002 John Wiley & Sons, Ltd.