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Description and validation of a staggered parallel high performance liquid chromatography system for good laboratory practice level quantitative analysis by liquid chromatography/tandem mass spectrometry
Author(s) -
King Richard C.,
MillerStein Cynthia,
Magiera Daniel J.,
Brann John
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.539
Subject(s) - chemistry , chromatography , mass spectrometry , high performance liquid chromatography , sample (material) , throughput , tandem mass spectrometry , liquid chromatography–mass spectrometry , tandem , analytical chemistry (journal) , computer science , telecommunications , materials science , composite material , wireless
The Aria LX4 staggered parallel high performance liquid chromatography (HPLC) system is evaluated for application to good laboratory practice (GLP) level quantitative analysis by liquid chromatography/tandem mass spectrometry (LC/MS/MS). This system consists of four fully independent binary HPLC pumps, a modified autosampler, and a series of switching and selector valves all controlled by a single computer program. The system improves sample throughput without sacrificing chromatographic separation or data quality. Validation results for four different compounds, each analyzed on a separate channel of the Aria system, show precision and accuracy equivalent to that required of a single‐channel system. The results show that sample throughput can be increased nearly four‐fold without requiring significant changes in current analytical procedures. The flexibility and ease of use of the Aria system suggest that it should be possible to quickly implement it in any analytical LC/MS/MS environment. Copyright © 2001 John Wiley & Sons, Ltd.