Premium
Gas chromatography/combustion/isotope ratio mass spectrometry to control the misuse of androgens in breeding animals: new derivatisation method applied to testosterone metabolites and precursors in urine samples
Author(s) -
Prévost Stéphanie,
Nicol Tatiana,
Monteau Fabrice,
André François,
Bizec Bruno Le
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.538
Subject(s) - chemistry , chromatography , mass spectrometry , urine , testosterone (patch) , isotope ratio mass spectrometry , gas chromatography , gas chromatography–mass spectrometry , combustion , endocrinology , organic chemistry , biochemistry , medicine
A new derivatisation reaction applied to the analysis of steroids by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) was studied. The trimethylsilylated steroids were characterised by well‐resolved chromatographic signals, no peak tailing, reproducible 13 C/ 12 C measurements (0.32‰, n = 28), good signal‐to‐noise ratio and absolute intensity (5 × 10 −9 A, 20 ng), and a slow degradation of copper oxide pellets in the combustion furnace. In addition, two new metabolites and one precursor of testosterone in bovine have been brought into consideration and used for GC/C/IRMS measurements, namely, 3β‐hydroxy‐5α‐androstan‐17‐one (epiandrosterone), 3β,17α‐dihydroxy‐5α‐androstane, and 3β,17α‐dihydroxy‐5‐androstene. The new findings have been applied to an elimination study in bovine of testosterone metabolites after an intramuscular injection of testosterone enanthate. Significant differences (up to 4‰) between testosterone metabolites and precursor were detectable at least three weeks after administration. Copyright © 2001 John Wiley & Sons, Ltd.