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Equal ratio of graphite carbon to activated charcoal for enrichment of N‐glycopeptides prior to matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometric identification
Author(s) -
Xin Ling,
Zhang Haijing,
Liu Hui,
Li Zhili
Publication year - 2012
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.5327
Subject(s) - chemistry , glycopeptide , chromatography , glycoproteomics , fetuin , matrix assisted laser desorption/ionization , activated carbon , mass spectrometry , monolith , carbon fibers , desorption , analytical chemistry (journal) , glycoprotein , biochemistry , adsorption , organic chemistry , composite material , composite number , catalysis , antibiotics , materials science
Site‐specific glycoprofiling of N‐glycoproteins is based on the enrichment of glycopeptides. In this study, we develop a simple and economical ZipTip microcolumn packed with a 1:1 ratio of graphite carbon to activated charcoal (w/w) (termed as GA‐ZipTip microcolumn) to efficiently isolate and enrich N‐glycopeptides from digested mixtures of individual glycoproteins and also investigate the ratio effect on the isolation and enrichment of glycopeptides. The experimental data showed that the microcolumn could efficiently and reproducibly isolate N‐glycopeptides from enzymatic mixtures of ovalbumin, fetuin, haptoglobin and human plasma. The results obtained here demonstrate that the GA‐ZipTip microcolumn is a powerful tool to enrich unbiasedly N‐glycopeptides, which will be benefit for glycoproteomics and complex biological samples. Copyright © 2012 John Wiley & Sons, Ltd.