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High‐performance thin‐layer chromatography plate blotting for liquid microjunction surface sampling probe mass spectrometric analysis of analytes separated on a wettable phase plate
Author(s) -
Walworth Matthew J.,
Stankovich Joseph J.,
Van Berkel Gary J.,
Schulz Michael,
Minarik Susanne
Publication year - 2011
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.5299
Subject(s) - chemistry , chromatography , analyte , mass spectrometry , analytical chemistry (journal) , electrospray , electrospray ionization , substrate (aquarium) , cellulose , thin layer chromatography , organic chemistry , oceanography , geology
A blotting method that transfers analytes separated on wettable high‐performance thin‐layer chromatography (HPTLC) plates to a hydrophobic reversed‐phase C8 HPLTC plate suitable for analysis with a liquid microjunction surface sampling probe electrospray ionization mass spectrometry system was described and demonstrated. The simple blotting procedure transfers the analyte from the wettable plate to the topmost surface of a rigidly backed, easy‐to‐mount hydrophobic substrate that already has been proven viable for analysis by this sampling probe/mass spectrometry system. The utility of the approach was demonstrated by the analysis of a four‐component peptide mixture originally separated on a ProteoChrom® HPTLC cellulose sheet and then blotted onto the reversed‐phase HPTLC plate. Published in 2011 by John Wiley & Sons, Ltd.