Premium
Proposal for a common nomenclature for peptide fragment ions generated following sequence scrambling during collision‐induced dissociation
Author(s) -
Chawner Ross,
Gaskell Simon J.,
Eyers Claire E.
Publication year - 2011
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.5294
Subject(s) - chemistry , queen (butterfly) , mass spectrometry , scrambling , dissociation (chemistry) , computer science , algorithm , organic chemistry , chromatography , botany , biology , hymenoptera
Figure 1. (A) The b5 ion is generated by collision induced dissociation resulting in formation of the oxazolone ring through attack of the oxygen lone pair on the newly formed terminal carbonyl group. (B) N-terminal nucleophilic attack of the oxazolone ring to form the macrocycle. (C) The resultant macrocyclic intermediate undergoes ring opening to give sequence scrambled b5 ions. N.B. Numbering of the peptide bonds is performed for the initial sequence and the order maintained throughout the scrambling pathway. This numbering system enables not only the scrambled product, but also the fragmentation pathway from which it was derived to be assigned. Bond 0 is that formed by N-terminal nucleophilic attack of the oxazolone ring. Ring opening at this position results in reformation of the original sequence. 20 Dear Editor,