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Molecular histology analysis by matrix‐assisted laser desorption/ionization imaging mass spectrometry using gold nanoparticles as matrix
Author(s) -
Tang HoWai,
Wong Melody YeeMan,
Lam Wing,
Cheng YungChi,
Che ChiMing,
Ng KwanMing
Publication year - 2011
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.5281
Subject(s) - mass spectrometry imaging , chemistry , mass spectrometry , matrix assisted laser desorption/ionization , colloidal gold , matrix (chemical analysis) , desorption , nanoparticle , analytical chemistry (journal) , chromatography , nanotechnology , materials science , adsorption , organic chemistry
Gold nanoparticles (AuNPs) were applied and optimized as matrix for matrix‐assisted laser desorption/ionization mass spectrometry analysis of animal tissues, and enabled histological analysis of animal tissues at molecular level by imaging mass spectrometry (IMS). AuNPs were coated on animal tissue in a solvent‐free manner via argon ion sputtering. Metabolites, including neurotransmitters, fatty acids and nucleobases, were directly detected from mouse brain tissue. Based on region‐specific chemical profiles, fine histological features of mouse brain tissue and heterogeneous regions of tumor tissue were both revealed. Copyright © 2011 John Wiley & Sons, Ltd.