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A discharge adaptor interface for use in liquid chromatography/mass spectrometry
Author(s) -
Sheen JennFeng,
Ho YaHui
Publication year - 2011
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.5278
Subject(s) - chemistry , atmospheric pressure chemical ionization , electrospray ionization , analytical chemistry (journal) , chromatography , mass spectrometry , solvent , ionization , adduct , interface (matter) , ion , chemical ionization , molecule , organic chemistry , gibbs isotherm
A discharge adaptor, composed of a metal casing and platinum (Pt) wire needle, was directly attached to an electrospray ionization (ESI) probe tip, to transform the ionization into atmospheric pressure chemical ionization (APCI). Six generic drugs were analyzed with the developed discharge adaptor (DA) and two commercial interfaces. The DA interface produced more intense radical anions, [M] .– , and less sodium adduct ions, [M + Na] + , than the ESI interface, whereas almost the same molecular ions were detected as the APCI interface. The effects of solvent and desolvation gas flow in the DA interface were similar to those in the ESI interface, but differed from those in the APCI interface. Better sensitivity of the tested drugs was obtained relative to the commercial APCI interface. For human plasma samples, the DA interface also demonstrated good tolerance to plasma matrices, linearity from 5 or 20 to 500 ng/mL (r 2 > 0.99) and ruggedness. Copyright © 2011 John Wiley & Sons, Ltd.