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Development of a simultaneous liquid chromatography/tandem mass spectrometric method for the determination of type B trichothecenes, their derivatives, and precursors in wheat
Author(s) -
Kadota Tomoyuki,
Kimura Makoto,
Hirano Satoshi,
Tajima Osamu,
Nakajima Takashi,
Kamata Yoichi,
SugitaKonishi Yoshiko
Publication year - 2011
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.5250
Subject(s) - chemistry , chromatography , tandem , tandem mass spectrometry , liquid chromatography–mass spectrometry , mass spectrometry , materials science , composite material
A method coupling liquid chromatography with tandem mass spectrometry (LC/MS/MS) was developed for the simultaneous quantitative determination of trichothecenes, nivalenol, deoxynivalenol, deoxynivalenol‐3‐glucoside, fusarenon‐X, 3‐acetyldeoxynivalenol, 15‐acetyldeoxynivalenol, isotrichodermin, calonectrin, 3‐deacetylcalonectrin, 15‐deacetylcalonectrin, 3,15‐diacetylnivalenol, 4,15‐diacetylnivalenol, 3,15‐diacetyldeoxynivalenol, and 3,4,15‐triacetylnivalenol. The analytical parameters of trichothecenes and their derivatives were optimized to enable their highly sensitive detection. Evaluation of clean‐up procedures using Multisep #226 and #227 indicated that Multisep #227 was more suitable for their simultaneous detection in wheat. In performance validation studies using the LC/MS/MS method with Multisep #227 cleanup, good recoveries ranging from 84% to 115% with relative standard deviations from 0.4% to 7.2% were measured. The limits of detection and quantification ranged from 0.03 to 1.4 ng·g −1 and from 0.1 to 4.7 ng·g −1 , respectively. The effect of matrices using matrix‐matched calibration was estimated to range from 80% to 117% after Multisep #227 cleanup. Multisep #227 clean‐up procedure with matrix‐free standard calibration achieved accurate quantification without having a considerable effect on matrix compounds. Using the developed method, several trichothecene derivatives and precursors were detected in fungally inoculated wheat samples. The developed LC/MS/MS method is a practical technique that can be used for the quantification of trichothecenes in wheat. This study is the first report of an analytical method used for the simultaneous quantification of major trichothecenes, their derivatives and precursors. Copyright © 2011 John Wiley & Sons, Ltd.