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Positive ion electrospray ionization mass spectrometry of double‐stranded DNA/drug complexes
Author(s) -
Gupta Rajesh,
Kapur Amit,
Beck Jennifer L.,
Sheil Margaret M.
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.524
Subject(s) - chemistry , electrospray ionization , dna , mass spectrometry , ion , ammonium acetate , ionization , electrospray , chromatography , mass spectrum , analytical chemistry (journal) , organic chemistry , biochemistry , high performance liquid chromatography
An Erratum has been published for this article in Rapid Communicatons in Mass Spectrometry 16(7) 2002,740–741. Positive ion electrospray ionization mass spectra of 16 base‐pair double‐stranded (ds)DNA have been obtained with essentially no ions from single‐stranded DNA present. Single‐stranded DNA was minimized by: (1) careful choice of DNA sequences; (2) the use of a relatively high salt concentration (0.1 M ammonium acetate, pH 8.5), and, (3) a low desolvation temperature (40 °C). Similarly, ESI‐MS complexes of dsDNA with cisplatin, daunomycin and distamycin were obtained that contained only negligible amounts of single‐stranded DNA. The complexes with daunomycin and distamycin were more stable to strand separation in the gas phase than dsDNA alone. This is in agreement with solution studies and with other recent gas phase results. These data contrast with many earlier ESI‐MS studies of dsDNA and DNA/drug complexes in which ions from ssDNA are also normally observed. Copyright © 2001 John Wiley & Sons, Ltd.