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Tissue‐spray ionization mass spectrometry for raw herb analysis
Author(s) -
Chan Sharon LaiFung,
Wong Melody YeeMan,
Tang HoWai,
Che ChiMing,
Ng KwanMing
Publication year - 2011
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.5177
Subject(s) - chemistry , ginseng , analyte , chromatography , ambient ionization , mass spectrometry , chemical ionization , herb , solvent , ion source , ionization , analytical chemistry (journal) , ion , medicinal herbs , organic chemistry , traditional medicine , alternative medicine , medicine , pathology
Tissue‐spray ionization mass spectrometry is developed for the in situ chemical analysis of raw herbs under ambient conditions. We demonstrated that analyte molecules could be directly sprayed and ionized from solvent‐wetted ginseng tissues upon the application of high electrical voltage to the tissue sample. Abundant phytochemicals/ metabolites, including ginsenosides, amino acids and oligosaccharides, could be detected from ginseng tissues when the tissue‐spray experiments were conducted in positive ion mode. Thermally labile and easily hydrolyzed malonyl‐ginsenosides were also detected in negative ion mode. The tissue‐spray ionization method enables the direct detection of analytes from raw herb samples and preserves the sample integrity for subsequent morphological and/ or microscopic examination. In addition, this method is simple and fast for chemical profiling of wild‐type and cultivated‐type American ginsengs with differentiation. Copyright © 2011 John Wiley & Sons, Ltd.