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Identification of sulfoquinovosyldiacyglycerides from Phaeodactylum tricornutum by matrix‐assisted laser desorption/ionization QTrap time‐of‐flight hybrid mass spectrometry
Author(s) -
Naumann Ivonne,
Klein Barbara C.,
Bartel Sebastian J.,
Darsow Kai H.,
Buchholz Rainer,
Lange Harald A.
Publication year - 2011
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.5137
Subject(s) - phaeodactylum tricornutum , chemistry , mass spectrometry , chromatography , desorption , time of flight mass spectrometry , matrix assisted laser desorption/ionization , matrix (chemical analysis) , ionization , organic chemistry , ion , botany , adsorption , biology , diatom
The pharmaceutical industry is interested in identifying novel target compounds. Due to their versatile pharmacological activities (e.g. antiviral, anti‐carcinogen and immunosuppressive) sulfoquinovosyldiacylglycerides (SQDGs) are potential drug candidates. The present publication deals with the purification and structural characterization of SQDGs from three different strains of Phaeodactylum tricornutum . Besides detection of SQDGs ( sn ‐1: C16:1/ sn ‐2: C16:0 and sn ‐1: C20:5/ sn ‐2: C16:0), two novel 2'‐ O ‐acylsulfoquinovosyldiacylglyerides (Ac‐SQDGs, sn ‐1: C16:0/ sn ‐2: C16:0/2' C20:5 and sn ‐1: C20:5/ sn ‐2: C16:0/2' C20:5) were identified by using matrix‐assisted laser desorption/ionization (MALDI) QTrap time‐of‐flight (ToF) hybrid mass spectrometry (MS) with multistage MS n . The analytical method enables the sn ‐position verification of fatty acids (MS 2 ) as well as the confirmation of the regioposition of eicospentanoic acid at the sulfoquinovose (MS 3 ). Copyright © 2011 John Wiley & Sons, Ltd.