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Liquid chromatography/electrospray ionisation mass spectrometric tracking of 4‐hydroxy‐2( E )‐nonenal biotransformations by mouse colon epithelial cells using [1,2‐ 13 C 2 ]‐4‐hydroxy‐2( E )‐nonenal as stable isotope tracer
Author(s) -
Jouanin I.,
Baradat M.,
Gieules M.,
Taché S.,
Pierre F. H. F.,
Guéraud F.,
Debrauwer L.
Publication year - 2011
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.5033
Subject(s) - chemistry , metabolite , mass spectrometry , chromatography , electrospray , electrospray ionization , in vivo , metabolism , metabolomics , hydroxylation , biochemistry , enzyme , microbiology and biotechnology , biology
4‐Hydroxy‐2( E )‐nonenal (HNE), a product of lipid peroxidation, has been extensively studied in several areas, including metabolism with radio‐isotopes and quantification in various matrices with deuterium‐labelled HNE as standard. The aim of this work was to evaluate the relevance of 13 C‐labelled HNE in biotransformation studies to discriminate metabolites from endogens by liquid chromatography/electrospray ionisation mass spectrometry (LC/ESI‐MS). 13 C‐Labelled HNE was synthesised in improved overall yield (20%), with the incorporation of two labels in the molecule. Immortalised mouse colon epithelial cells were incubated with 2:3 molar amounts of HNE/ 13 C‐HNE in order to gain information on the detection of metabolites in complex media. Our results demonstrated that the stable isotope m/z values determined by mass spectrometry were relevant in distinguishing metabolites from endogens, and that metabolite structures could be deduced. Six conjugate metabolites and 4‐hydroxy‐2( E )‐nonenoic acid were identified, together with an incompletely identified metabolite. Stable‐isotope‐labelled HNE has already been used for quantification purposes. However, this is the first report on the use of 13 C‐labelled HNE as a tracer for in vitro metabolism. 13 C‐Labelled HNE could also be of benefit for in vivo studies. Copyright © 2011 John Wiley & Sons, Ltd.