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Multi‐stage mass spectrometric information obtained by deconvolution of energy‐resolved spectra acquired by triple‐quadrupole mass spectrometry
Author(s) -
Kanie Osamu,
Kanie Yoshimi,
Daikoku Shusaku,
Shioiri Yuki,
Kurimoto Ayako,
Mutsuga Sachiko,
Goto Satoshi,
Ito Yukishige,
Suzuki Katsuhiko
Publication year - 2011
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.5031
Subject(s) - chemistry , deconvolution , mass spectrometry , triple quadrupole mass spectrometer , analytical chemistry (journal) , quadrupole , mass spectrum , selected reaction monitoring , chromatography , atomic physics , tandem mass spectrometry , physics , optics
Triple‐quadrupole mass spectrometry (TQ‐MS) provides the capability to carry out collision‐induced dissociation (CID) and it offers advantages in quantification when connected with high‐performance liquid chromatography through an electrospray ionization interface. However, although TQ‐MS provides information on partial structures through the analysis of product ions obtained by CID experiments, the method only provides single‐stage CID experiments, which limits the detailed structural information that can be obtained. Herein, a method of overcoming this limitation of TQ‐MS is described. A spectrum obtained by energy‐resolved mass spectrometry (ERMS) was used to deconvolute the fragmentation process, with a Galili‐antigenic trisaccharide derivative being used as an example. A replot of the ERMS data showing the ratios of the product ions to the precursor ion resulted in a descriptive graph. Analysis of the sum of the ratios of individual product ions to the precursor ion at specific CID energies revealed that the members of a series of product ions were related to each other. The obtained relationships and the m/z values of the product ions provided information on the fragmentation process taking place during the dissociation, indicating that the ERMS spectrum obtained by TQ‐MS contained equivalent information to that obtainable by multi‐stage MS/MS (MS n ; n ≥ 2). This method may allow users of triple‐quadrupole mass spectrometers to obtain MS n ‐type information by performing a single ERMS experiment, which is even advantageous over quadrupole ion trap (QIT)‐MS/MS because CID experiments on individual first‐generation product ions are not required. Copyright © 2011 John Wiley & Sons, Ltd.