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On‐line reaction monitoring by extractive electrospray ionisation
Author(s) -
McCullough Bryan J.,
Bristow Tony,
O'Connor Gavin,
Hopley Chris
Publication year - 2011
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.5016
Subject(s) - chemistry , electrospray , extractive electrospray ionization , electrospray ionization , chromatography , analytical chemistry (journal) , mass spectrometry , sample preparation in mass spectrometry
The design and development of a novel extractive electrospray ionisation (EESI) device for on‐line reaction monitoring is described. The EESI apparatus uses a secondary, grounded nebuliser to produce an analyte aerosol and a Venturi pump is then used to transfer a sample of the aerosol to an electrospray source where it is ionised. The EESI apparatus was then tested with a variety of small, organic molecules to assess sensitivity, linearity and dynamic range. The performance of the technique will depend on the mass spectrometer used for the experiments; in the configurations used here it has a useable dynamic range of around 3.5 orders of magnitude with a linear range of around 2.5 orders of magnitude and is capable of analysing species present down to low µg/mL with signal‐to‐noise ratio greater than 2.5. The use of EESI for reaction monitoring was validated using a series of mock reaction mixtures and then used to monitor the base hydrolysis of ethyl salicylate to salicylic acid. Copyright © 2011 John Wiley & Sons, Ltd.