Determination of aflatoxins in animal feeds by liquid chromatography/tandem mass spectrometry with isotope dilution | Zendy

Li Wei | Zendy; Herrman Timothy J. | Zendy; Dai Susie Y. | Zendy

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Determination of aflatoxins in animal feeds by liquid chromatography/tandem mass spectrometry with isotope dilution

Author(s) -

Li Wei,

Herrman Timothy J.,

Dai Susie Y.

Publication year - 2011

Publication title -

rapid communications in mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.528

H-Index - 136

eISSN - 1097-0231

pISSN - 0951-4198

DOI - 10.1002/rcm.4979

Subject(s) - chemistry , chromatography , isotope dilution , aflatoxin , mass spectrometry , tandem mass spectrometry , liquid chromatography–mass spectrometry , extraction (chemistry) , matrix (chemical analysis) , dilution , solid phase extraction , chromatographic separation , high performance liquid chromatography , food science , physics , thermodynamics

The objective of the present study is to develop a simple, fast method for detection of aflatoxins in animal feeds. Simultaneous quantitation of four aflatoxins (AFB 1 , AFB 2 , AFG 1 and AFG 2 ) in animal feeds was achieved in a single liquid chromatography/tandem mass spectrometry (LC/MS/MS) run. The solid‐phase extraction cleanup step is eliminated with the stable isotope dilution method. Matrix effects were observed and overcome by isotope dilution. The method was tested in a variety of animal feed matrices and proved to be accurate and reliable. Method ruggedness tests resulted in recoveries of 78% to 122% with an intra‐day assay precision of 2% to 15% and an inter‐day assay precision of 3% to 17%. These results indicate that this method is suitable for quantitation of aflatoxins in animal feeds. Copyright © 2011 John Wiley & Sons, Ltd.

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